Cryopreservation of Nili-Ravi buffalo bull semen: yearly postthaw analyses to depict climatic effects

The present study was aimed to observe the postthaw quality of Nili-Ravi buffalo bull semen cryopreserved during heat stress (hot summer months) and normal climatic conditions (fall, winter, spring months). For this purpose, four (n = 4) adult regular donor bulls were selected collect cryopreserve their throughout period an entire year. cryopreservation done following standard freezing protocol. doses condition thawed determine sperm motility, motion kinetics, viability, plasma membrane integrity, acrosomal morphological abnormalities (head, mid-piece, tail). results showed that motility kinetics did not alter (p > 0.05) across period. head, mid-piece tail differ 0.05); however, total morphologic higher < period, whereas lower membrane, integrity observed In conclusion, abnormalities, hot months are compromised due stress.